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protoarray human protein microarray v5.0 kinase substrate identification complete kit  (Thermo Fisher)


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    Thermo Fisher protoarray human protein microarray v5.0 kinase substrate identification complete kit
    <t>Microarray</t> screen for FAK substrates. a. Left: Autoradiograph image of control and FAK protein microarray slides. Right: Control microarray containing 1, 5, 10 and 25 ng/dot of GST or GST-Paxillin. b. Spot-pair signals from control and FAK slides block #40 (upper panels), with identified proteins, microarray positions and relative signal intensities (average pixels/pair) below. A-F, proteins with increased phosphorylation by FAK relative to control; G, positive control protein, PKCeta; H-J , proteins with equal or decreased phosphorylation levels in the FAK slide compared to control.
    Protoarray Human Protein Microarray V5.0 Kinase Substrate Identification Complete Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoarray human protein microarray v5.0 kinase substrate identification complete kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    protoarray human protein microarray v5.0 kinase substrate identification complete kit - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Identification of Novel Focal Adhesion Kinase Substrates: Role for FAK in NFκB Signaling"

    Article Title: Identification of Novel Focal Adhesion Kinase Substrates: Role for FAK in NFκB Signaling

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.10273

    Microarray screen for FAK substrates. a. Left: Autoradiograph image of control and FAK protein microarray slides. Right: Control microarray containing 1, 5, 10 and 25 ng/dot of GST or GST-Paxillin. b. Spot-pair signals from control and FAK slides block #40 (upper panels), with identified proteins, microarray positions and relative signal intensities (average pixels/pair) below. A-F, proteins with increased phosphorylation by FAK relative to control; G, positive control protein, PKCeta; H-J , proteins with equal or decreased phosphorylation levels in the FAK slide compared to control.
    Figure Legend Snippet: Microarray screen for FAK substrates. a. Left: Autoradiograph image of control and FAK protein microarray slides. Right: Control microarray containing 1, 5, 10 and 25 ng/dot of GST or GST-Paxillin. b. Spot-pair signals from control and FAK slides block #40 (upper panels), with identified proteins, microarray positions and relative signal intensities (average pixels/pair) below. A-F, proteins with increased phosphorylation by FAK relative to control; G, positive control protein, PKCeta; H-J , proteins with equal or decreased phosphorylation levels in the FAK slide compared to control.

    Techniques Used: Microarray, Autoradiography, Blocking Assay, Positive Control



    Similar Products

    protoarray human protein microarray v5.0 kinase substrate identification complete kit  (Thermo Fisher)
    90
    Thermo Fisher protoarray human protein microarray v5.0 kinase substrate identification complete kit
    <t>Microarray</t> screen for FAK substrates. a. Left: Autoradiograph image of control and FAK protein microarray slides. Right: Control microarray containing 1, 5, 10 and 25 ng/dot of GST or GST-Paxillin. b. Spot-pair signals from control and FAK slides block #40 (upper panels), with identified proteins, microarray positions and relative signal intensities (average pixels/pair) below. A-F, proteins with increased phosphorylation by FAK relative to control; G, positive control protein, PKCeta; H-J , proteins with equal or decreased phosphorylation levels in the FAK slide compared to control.
    Protoarray Human Protein Microarray V5.0 Kinase Substrate Identification Complete Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoarray human protein microarray v5.0 kinase substrate identification complete kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    protoarray human protein microarray v5.0 kinase substrate identification complete kit - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Microarray screen for FAK substrates. a. Left: Autoradiograph image of control and FAK protein microarray slides. Right: Control microarray containing 1, 5, 10 and 25 ng/dot of GST or GST-Paxillin. b. Spot-pair signals from control and FAK slides block #40 (upper panels), with identified proteins, microarray positions and relative signal intensities (average pixels/pair) below. A-F, proteins with increased phosphorylation by FAK relative to control; G, positive control protein, PKCeta; H-J , proteins with equal or decreased phosphorylation levels in the FAK slide compared to control.

    Journal: International Journal of Biological Sciences

    Article Title: Identification of Novel Focal Adhesion Kinase Substrates: Role for FAK in NFκB Signaling

    doi: 10.7150/ijbs.10273

    Figure Lengend Snippet: Microarray screen for FAK substrates. a. Left: Autoradiograph image of control and FAK protein microarray slides. Right: Control microarray containing 1, 5, 10 and 25 ng/dot of GST or GST-Paxillin. b. Spot-pair signals from control and FAK slides block #40 (upper panels), with identified proteins, microarray positions and relative signal intensities (average pixels/pair) below. A-F, proteins with increased phosphorylation by FAK relative to control; G, positive control protein, PKCeta; H-J , proteins with equal or decreased phosphorylation levels in the FAK slide compared to control.

    Article Snippet: The following reagents/kits were used: ProtoArray Human Protein Microarray v5.0 Kinase Substrate Identification Complete Kit (Invitrogen), full-length active Src (SignalChem), GST-FAK (Invitrogen), baculovirus-encoded Pyk2 (gift of Vita Golubovskaya, Roswell Park Cancer Institute [RPCI]), purified vitronectin (Advanced BioMatrix), purified GST-PTPN5[a.a.17-565] (ProteinTech), FAK inhibitor PF-573,228 (Sigma), TNFα (Abcam), Recombinant Light (Enzo Life Sciences).

    Techniques: Microarray, Autoradiography, Blocking Assay, Positive Control